Pular para o conteúdo
Merck
  • Chiral amine synthesis using ω-transaminases: an amine donor that displaces equilibria and enables high-throughput screening.

Chiral amine synthesis using ω-transaminases: an amine donor that displaces equilibria and enables high-throughput screening.

Angewandte Chemie (International ed. in English) (2014-08-21)
Anthony P Green, Nicholas J Turner, Elaine O'Reilly
RESUMO

The widespread application of ω-transaminases as biocatalysts for chiral amine synthesis has been hampered by fundamental challenges, including unfavorable equilibrium positions and product inhibition. Herein, an efficient process that allows reactions to proceed in high conversion in the absence of by-product removal using only one equivalent of a diamine donor (ortho-xylylenediamine) is reported. This operationally simple method is compatible with the most widely used (R)- and (S)-selective ω-TAs and is particularly suitable for the conversion of substrates with unfavorable equilibrium positions (e.g., 1-indanone). Significantly, spontaneous polymerization of the isoindole by-product generates colored derivatives, providing a high-throughput screening platform to identify desired ω-TA activity.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
o-Xylylenediamine dihydrochloride, ≥98%