- Overcoming the challenges of membrane protein crystallography.
Overcoming the challenges of membrane protein crystallography.
Current opinion in structural biology (2008-08-05)
Elisabeth P Carpenter, Konstantinos Beis, Alexander D Cameron, So Iwata
PMID18674618
RESUMO
Membrane protein structural biology is still a largely unconquered area, given that approximately 25% of all proteins are membrane proteins and yet less than 150 unique structures are available. Membrane proteins have proven to be difficult to study owing to their partially hydrophobic surfaces, flexibility and lack of stability. The field is now taking advantage of the high-throughput revolution in structural biology and methods are emerging for effective expression, solubilisation, purification and crystallisation of membrane proteins. These technical advances will lead to a rapid increase in the rate at which membrane protein structures are solved in the near future.
MATERIAIS