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Merck

An ELISA test for detecting chloroquine in urine.

Transactions of the Royal Society of Tropical Medicine and Hygiene (1988-01-01)
F C Shenton, M Bots, A Menon, T A Eggelte, M de Wit, B M Greenwood
RESUMO

Two ELISA tests for detecting chloroquine in urine have been developed using polyclonal and monoclonal antibodies which react with the 7-chloro-4-amino-quinoline part of the chloroquine molecule and thus recognize chloroquine, its metabolites, and amodiaquine. The ELISAs were sensitive and specific and did not cross-react with other commonly used antimalarials. In a field trial the chloroquine ELISA performed better than the Dill Glazko or Haskins colorimetric tests. A small proportion of urines gave an apparently false positive reaction when tested at a dilution of 1:10, but not when tested at higher dilution.

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Sigma-Aldrich
4-Amino-7-chloroquinoline, AldrichCPR
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