- Linchpins empower promiscuous electrophiles to enable site-selective modification of histidine and aspartic acid in proteins.
Linchpins empower promiscuous electrophiles to enable site-selective modification of histidine and aspartic acid in proteins.
Chemical science (2021-05-28)
Dattatraya Gautam Rawale, Kalyani Thakur, Pranav Sreekumar, Sajeev T K, Ramesh A, Srinivasa Rao Adusumalli, Ram Kumar Mishra, Vishal Rai
PMID34040749
RESUMO
The conservation of chemoselectivity becomes invalid for multiple electrophilic warheads during protein bioconjugation. Consequently, it leads to unpredictable heterogeneous labeling of proteins. Here, we report that a linchpin can create a unique chemical space to enable site-selectivity for histidine and aspartic acid modifications overcoming the pre-requisite of chemoselectivity.
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Millipore
Immobilon®-PSQ Membrane, PVDF, 0.2 m, 8.5 cm x 10 m roll, 0.2 um filter ( 0.2 micron ) pore size Hydrophobic PVDF Transfer Membrane for western blotting using Chemiluminescence, Chromogenic or Radioactive detection methods. Comes in an 8.5 cm x 10 m roll.