The localization of 2':3'-cyclic nucleotide 3'-phosphodiesterase in bovine cerebrum by immunofluorescence.

The distribution of 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNP) in fixed-tissue sections and Percoll-isolated cells (oligodendrocytes) of bovine cerebrum was studied by immunofluorescent techniques. Fluorescence in tissue sections exposed to CNP antisera was restricted to oligodendrocyte-like cells. Also, Percoll-isolated cells (oligodendrocytes) were positively labeled with CNP antisera. This study has shown that CNP may be used as an antigenic marker for oligodendrocytes.