Tris-Buffered Saline (TBS) 10X Stock Solution for Western Blots

Tris-buffered saline (TBS) is an excellent wash buffer for many types of immunoassays. To make 1 L of 10X TBS stock solution, dissolve 24 g Tris and 88 g NaCl in 900 mL of water and then adjust the pH to 7.6 and final volume to 1 L.

This calculator enables the preparation of a 10X TBS wash buffer stock solution, whether you are preparing enough for a single experiment or for the entire lab. Input your desired volume and click the CALCULATE button. The right side of the table will populate with the correct amounts of each reagent needed for your desired total final volume.

Solution concentrations:

• Tris: 200 mM
• NaCl: 1500 mM

OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS & SOLUTIONS

Stock Solutions

• 4X SDS Sample Loading Buffer
• 10X Phosphate-Buffered Saline (PBS)
• Stock Components for Western Blot Stripping Solutions

Wash & Blocking Buffers

• 1X Tris-Buffered Saline (TBS) Working Solution
• 1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST)
• 1X Phosphate-Buffered Saline (PBS) Recipe Calculator
• 1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST)
• Non-Fat Dry Milk (NFDM) Blocking Buffer
• Bovine Serum Albumin (BSA) Blocking Buffer

Stripping Solutions

• Detergent-based Western Blot Stripping Buffer
• Acidic Western Blot Stripping Solution
• Stock Components for Western Blot Stripping Solutions