MABC684

Anti-SOD1 Antibody, clone 6F5

ascites fluid, clone 6F5, from mouse

• Sinônimo(s): ALS, SOD, ALS1, IPOA, homodimerSuperoxide dismutase [Cu-Zn], Superoxide dismutase 1, hSod1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: ascites fluid
• tipo de produto de anticorpo: primary antibodies
• clone: 6F5, monoclonal
• reatividade de espécies: mouse, human
• técnica(s): flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG1
• nº de adesão UniProt: P00441
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... SOD1(6647)

Descrição

Descrição geral

Superoxide dismutase 1 (SOD1) also known as Superoxide dismutase [Cu-Zn] to distinguish it from SOD2 which uses manganese as its cofactor, is the cytosolic form of the enzyme that catalyzes the breakdown of superoxide into oxygen and hydrogen peroxide and is our primary anti-oxidant combating enzyme. SOD1 performs its function with the help of co-factors and one protein in particular is vital to its proper functioning. Copper chaperone protein (CCS) is a metalloprotein that specifically delivers Cu to SOD1 and may activate SOD1 through direct insertion of the Cu cofactor. Interestingly, defects in SOD1 are the cause of a form of ALS (Amyotrophic Lateral Sclerosis or Lou Gehrig′s disease) and much research is centered on understanding how over-active or under-active SOD1 contributes to the disease. EMD-Millipore′s Anti-SOD1 mouse monoclonal antibody has been tested in western blot on HeLa, NIH3T3, A549 and A431 cell lysates and fluorescent immunocytochemistry on PANC1, SKBR-3 and 3T3-L1 cells in culture. The antibody has also been tested in flow cytometry on A431 cells in culture.

Imunogênio

Purified recombinant fragment of human SOD1 expressed in E. Coli.

Aplicação

Anti-SOD1 Antibody, clone 6F5 is a highly specific mouse monoclonal antibody, that targets Superoxide Dismutase & has been tested in western blotting, ICC & Flow Cytometry.

Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected SOD1 in PANC-1, SKBR-3, and 3T3-L1 cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SOD1 in non serum starved A431 cells.

Optimal working dilutions must be determined by end user.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Qualidade

Evaluated by Western Blotting in HeLa, NIH/3T3, A549, and A431 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SOD1 in HeLa, NIH/3T3, A549, and A431 cell lysates.

Descrição-alvo

~18 kDa observed. Uncharacterized bands may appear in some lysate(s).

forma física

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análise

Control
HeLa, NIH/3T3, A549, and A431 cell lysates

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable