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Sigma-Aldrich

Anti-Histone H3, Acetylated (1-20) Rabbit pAb

liquid, Calbiochem®

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100 μG
₹26,450.45

₹26,450.45


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100 μG
₹26,450.45

About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

₹26,450.45


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fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

Formulário

liquid

contém

≤0.1% sodium azide as preservative

reatividade de espécies

Tetrahymena sp., human

fabricante/nome comercial

Calbiochem®

condição de armazenamento

OK to freeze
avoid repeated freeze/thaw cycles

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Este Item
SAB4503475ABE1859ABF206
Anti-TIF1-alpha Antibody from rabbit, purified by affinity chromatography

ABE434

Anti-TIF1-alpha Antibody

Anti-KAP-1/TRIM28 Antibody serum, from rabbit

ABE1859

Anti-KAP-1/TRIM28 Antibody

Anti-TRIM5 alpha Antibody from rabbit, purified by affinity chromatography

ABF206

Anti-TRIM5 alpha Antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

serum

antibody form

affinity isolated antibody

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

species reactivity

mouse, human

species reactivity

mouse, human

species reactivity

mouse, human

species reactivity

human

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

Descrição geral

Anti-Histone H3, Acetylated (1-20), rabbit polyclonal, recognizes the ~17 kDa acetylated histone H3 protein. It is validated for use in Western blotting, immunocytochemistry & immunoprecipitation.
Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa acetylated histone H3 protein.
Recognizes the ~17 kDa acetylated histone H3 protein.

Imunogênio

Human
a diacetylated synthetic peptide [ARTKQTARAcKSTGGAcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH

Aplicação

Immunoblotting (10-500 ng/ml, chemiluminescence)

Immunocytochemistry (5 µg/ml)

Immunoprecipitation (see comments)

Embalagem

Please refer to vial label for lot-specific concentration.

Advertência

Toxicity: Standard Handling (A)

forma física

In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.

Reconstituição

Following initial thaw, aliquot and freeze (-20°C).

Nota de análise

Positive Control
HeLa cells

Outras notas

Braunstein, M., et al. 1996. Mol. Cell. Biol.16, 4349.
Braunstein, M., et al. 1993. Genes Devel.7, 592.
Perry, C.A., et al. 1993. Biochemistry32, 13605.
Lin, R., et al. 1989. J. Cell. Biol.108, 1577.
For immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser10 on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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