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Key Documents

G005803

LC/MS Extracted Ion Chromatogram of MSX, Sulfone, and Sulfoxide on Ascentis® Express OH5 (HILIC Mode)

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express OH5, 10 cm x 3 mm I.D., 2.7 μm particles (53769-U)
mobile phase: [A] 0.1% formic acid, pH 3.5 with ammonium formate; [B] acetonitrile; (25:75, A:B)
flow rate: 0.4 mL/min
pressure: 1350 psi (93 bar)
column temp.: ambient
detector: ESI (+), scan m/z 150-300
injection: 5 μL
sample: 10 μg/mL in 90% methanol

suitability

application for HPLC

application(s)

chemicals and industrial polymers

Analysis Note

This figure shows the extracted ion currents for methionine sulfoximine-related compounds on Ascentis Express OH5. The OH5 shows excellent peak shape compared to traditional HILIC on bare silica.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    L-Methionine sulfoximine, suitable for cell culture

analytical column

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