Skip to Content
Merck
All Photos(1)

Key Documents

G005458

HPLC Analysis of mAb Charge Variants on TSKgel® CM-STAT

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable

test parameters

column: TSKgel CM-STAT, 10 cm x 4.6 mm I.D., 7 μm particles (821966)
mobile phase: [A] 20 mM MES buffer, pH 6.0; [B] 0.5 M NaCl in buffer A, pH 6.0
gradient: 0% B (0 min), 30% B (15 min), 100% B (15 min),

0% B (17 min), 10% B (17 min), 10% B (21 min)
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 280 nm
injection: 20 μL
sample: monoclonal antibodies (mAb A through E)

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

A TSKgel CM-STAT weak cation exchange (WCX) column was applied to separate charge variants of several monoclonal antibodies. The typical analysis time on conventional 25 cm long WCX columns of about eighty minutes could be significantly reduced when separation was performed on a 10 cm TSKgel CM-STAT column, filled with 7 μm particles. The analysis profiles for five antibodies show that high resolution analysis can be obtained in about 20 minutes analysis time.

Citation

From "Ion Exchange Chromatography for the Characterization of Biotherapeutics" in The Supelco Reporter, Vol. 29.3, page 20.

Legal Information

TSKgel is a registered trademark of Tosoh Corporation

analytical column

Product No.
Description
Pricing

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service