G004432
HPLC Analysis of Statins in Rat Plasma on Ascentis® Express C18 after SPE using Supel™-Select HLB
application for SPE, application for LC-MS
About This Item
technique(s)
LC/MS: suitable
solid phase extraction (SPE): suitable
test parameters
sample preparation: SPE (Solid Phase Extraction)
sample/matrix: rat plasma spiked with provastin and atorvastin at 5 and 100 ng/mL
SPE tube/cartridge: Supel-Select HLB SPE, 30 mg/1 mL (54181-U)
condition: 0.5 mL methanol:acetonitrile (1:1, v/v); 0.5 mL DI water
sample addition: 0.5 mL
washing: 0.5 mL 5% methanol
elution: 0.5 mL methanol:acetonitrile (1:1, v/v)
column: Ascentis Express C18, 5 cm x 2.1 mm, 2.7 μm particles (53822-U)
eluate post-treatment: evaporate to dryness with nitrogen at 37 °C, reconstitute in 0.5 mL DI water
mobile phase: 0.1% acetic acid diluted in 60% methanol
flow rate: 0.2 mL/min
column temp.: 35 °C
detector: ABI 3200 QT; ESI(+), MRM (423.3/321.3, 423.3/101.0, 557.3/397.2 and 557.3/453.4)
injection: 5 μL
suitability
application for LC-MS
application for SPE
application(s)
clinical
Legal Information
analytical column
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