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816260

TSKgel® Phenyl-5PW RP HPLC Column

phase phenyl, L × I.D. 15 cm × 21.5 mm, 13 μm particle size

Synonym(s):

TSKgel® Reversed Phase HPLC Column (Polymer-based)

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About This Item

UNSPSC Code:
41115711
eCl@ss:
32110501

material

stainless steel hardware

product line

TSKgel®

manufacturer/tradename

Tosoh Phenyl-5PW, 16260

extent of labeling

1 meq/mL ligand density (estimate)

parameter

no limitation salt concentration
0-100% organic solvent
30 bar max. pressure
4-60 °C temperature
8 mL/min flow rate

technique(s)

HPLC: suitable

L × I.D.

15 cm × 21.5 mm

matrix

polymer particle platform

matrix active group

phenyl phase

particle size

13 μm

pore size

1000 Å

operating pH

2-12

separation technique

reversed phase

General description

TSKgel Phenyl-5PW RP offers similiar selectivity to its hydrophobic interation counterpart TSKgel Phenyl-5PW, although a higher degree of cross-linking allows for increased stability in aqueous/organic mobile phase gradients. The highly cross-linked polymethacrylate base material provides an advantage over silica when high pH buffer systems are needed. Additionally, TSKgel Phenyl-5PW RP can withstand rigorous cleaning protocols using either acid or base. The 1000Å pore size accommodates globular protein samples up to 1,000,000 Da.
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
  • Octadecyl-2PW particles have 125Å pores
  • Octadecyl-4PW particles have 500Å pores
  • Phenyl-5PW RP particles have 1000Å pores
  • Octadecyl-NPR particles are nonporous

Physical form

Shipped in 30% methanol - 70% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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