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54182-U

Supelco

Supel™-Select HLB SPE Tube

bed wt. 60 mg, volume 3 mL, pk of 50

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About This Item

UNSPSC Code:
41115712
NACRES:
NB.21

material

PE frit (20 μm)
polypropylene hardware

quality

MS friendly

composition

bed wt., 60 mg

packaging

pk of 50

technique(s)

solid phase extraction (SPE): suitable

surface area

160-420 m2/g

volume

3 mL

matrix

Hydrophilic modified styrene polymer particle platform

matrix active group

polystyrene phase

particle size

50-70 μm

pore size

80-200 Å pore size

application(s)

food and beverages

separation technique

reversed phase

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General description

Retention Mechanism: Reversed-phase
Sample Matrix Compatibility: Aqueous solutions (biological fluids, water)

  • Hydrophilic modified styrene polymer
  • Developed for the extraction of a highly broad range of compounds from aqueous samples
  • Retention predominately based on reversed-phase; however, because the phase is hydrophilic modified, it is amenable to polar compounds as well.
  • Examples of more polar compounds that are retained and recovered include (but not limited to): pyridoxine (logPo/w -0.56), riboflavin (logPo/w -2.02), biotin (logPo/w 0.11).

Application

Supel™-Select HLB SPE Tube may be used as an extraction cartridge for the solid phase extraction and determination of antibiotics in water samples using liquid chromatography electrospray ionization tandem mass spectrometry.

Features and Benefits

  • Excellent sample prep performance at a lower price
  • Amenable to generic methodology - save time, money, and headache during method development
  • Greater capacity allows for smaller bed weights = smaller elution volumes = time savings in sample processing
  • Resistance to overdrying allows for more robust methdology
  • Low UV and MS extractables for lower background and greater sensitivity
  • Stringent production and QC guidelines offer greater lot-to-lot, tube-to-tube, and well-to-well consistency for improved accuracy and precision

Legal Information

Supel is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
14827901
14698601
14519801
14428101
14334701

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Analysis of selected antibiotics in surface freshwater and seawater using direct injection in liquid chromatography electrospray ionization tandem mass spectrometry
Bayen S, et al.
Journal of Chromatography A, 1338, 38-43 (2014)
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Articles

Supel-Select HLB SPE for Pharmaceutical Analysis

Select HLB SPE is a hydrophilic polymer phase for analyte extraction from aqueous matrices. Here it’s used for statins in plasma and 48 pharmaceutical analytes in drinking water.

Solid Phase Extraction: Reversed-Phase Methodology

Reversed-phase interaction will retain most molecules with hydrophobic character; it is very useful for extracting analytes that are very diverse in structure within the same sample.

Protocols

LC MS Analysis of Triclosan Irgasan in Waste Water

Separation of Irgasan, ≥97.0% (HPLC)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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