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description
Silica Column Regeneration Solution
packaging
pkg of 200 mL
compatibility
Restoring Column Performance
storage temp.
room temp
General description
Silica Column Regeneration Solution contains a mixture of acetic acid, dimethoxypropane, and methylene chloride.
This solution effectively regenerates a silica column that has come into contact with very strongly polar solvents, such as water or alcohols. Simply flush the column with regeneration solution for 10 minutes, then reequilibrate with mobile phase for 10 minutes. Column performance usually is restored to that obtained before exposure to the polar solvent.
Application
It was used to flush and regenerate the column used for HPLC analysis of tocopherols and tocotrienols.
Analyte
Description
Acetic acid
Dichloromethane
2,2-Dimethoxypropane
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Central nervous system
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Simple solutions to problems encountered in quantitative analysis of tocopherols and tocotrienols using silica columns.
A K Hewavitharana
Analytical biochemistry, 313(2), 342-344 (2003-02-28)
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