ZRB1977

Anti-ERK5 Antibody, clone 2H15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): BMK-1, Big MAP kinase 1, EC:2.7.11.24, Extracellular signal-regulated kinase 5, MAP kinase 7, MAPK 7, Mitogen-activated protein kinase 7
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2H15, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• purified by: using Protein A
• species reactivity: human, mouse
• species reactivity (predicted by homology): monkey, porcine, canine, bovine, feline
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): ELISA: suitable; affinity binding assay: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminus
• Protein ID accession no.: NP_002740
• UniProt accession no.: Q13164
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2H15 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects ERK5. It targets an epitope within 18 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human ERK5.

Application

Quality Control Testing

Evaluated by Western Blotting in NIH3T3 cell nuclear extract.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected ERK5 in NIH3T3 cell nuclear extract.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected ERK5 in human spleen tissue sections.

Affinity Binding Assay:: A representative lot of this antibody bound ERK5 peptide with a KD of 1.3 x 10-8 in an affinity binding assay.

Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected recombinant Human ERK5.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GST-tagged recombinant Human ERK5.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected ERK5 in one million A549 and HeLa cells.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected ERK5 in Human spleen tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Mitogen-activated protein kinase 7 (UniProt: Q13164; also known as EC:2.7.11.24, MAP kinase 7, MAPK 7, Big MAP kinase 1, BMK-1, Extracellular signal-regulated kinase 5, ERK-5) is encoded by the MAPK7 (also known as BMK1, ERK5, PRKM7) gene (Gene ID: 5598) in human. ERK5 is an unusual protein kinase with a kinase domain (aa 55-347) and a transcriptional transactivation domain (aa 664-789). It is expressed in many adult tissues and higher expression is observed in heart, placenta, lung, kidney, and skeletal muscle. Although its kinase domain  displays high sequence identity with ERK1/2, they tend to have distinct substrates and unlike ERK1/2, it has a large C-terminal extension that contains a NuCLEAR^™ localization signal (aa 505-539) and the transcriptional transactivation domain. ERK5 plays a role in various cellular processes, including cell proliferation, differentiation, and cell survival. Its upstream activator is MAP2K5 and following activation, it translocates to the nucleus and phosphorylates various downstream targets. It is activated by Dual^® phosphorylation of threonine 219 and tyrosine 221. ERK5 is also important for endothelial function and maintenance of blood vessel integrity. ERK5-deficient mice die around embryonic day 10.5 because of cardiovascular defects and angiogenic failure in embryonic and extraembryonic tissues.  Four isoforms of ERK5 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: (Ref.: Cook, SJ., and Lochhead, PA. (2022). Front. Cell Dev. Biol. 10; 839997; Yang, Q., et al. (2013). Oncogene. 32(26); 3156-3264; Nishimoto, S., and Nishida, E.  (2006). EMBO Rep. 7(8); 782-786).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Affinity is a trademark of Mine Safety Appliances Co.

Dual is a registered trademark of Ciba-Geigy AG

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable