ZRB1311
Anti-LRP1 85kDa subunit Antibody, clone 4G7, ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
A2MR, APOER, Alpha-2-macroglobulin receptor, Apolipoprotein E receptor, CD91, LRP-1 85 kDa subunit, Prolow-density lipoprotein receptor-related protein 1
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
rabbit (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
4G7, monoclonal
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
species reactivity
rat, mouse, human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... LRP1(4035)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 4G7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects the 85 kDa subunit of LRP-1. It targets an epitope within the C-terminal cytoplasmic domain.
Immunogen
His-tagged recombinant fragment corresponding to 100 amino acids from the C-terminal, cytoplasmic domain of human Prolow-density lipoprotein receptor-related protein 1 (LRP-1).
Application
Anti-LRP1 85kDa subunit, clone 4G7 ZooMAb, Cat. No. ZRB1311, is a recombinant rabbit monoclonal antibody that specifically detects LRP1 85kDa subunit and has been tested for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LRP1 85 kDa subunit in HEK293 cell lysate.
Flow Cytometry Analysis: 1 μg from a representative lot detected LRP1 85kDa subunit in one million HEK293 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LRP1 85kDa subunit in C6, PC12, C2C12, SH-SY5Y cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Flow Cytometry Analysis: 1 μg from a representative lot detected LRP1 85kDa subunit in one million HEK293 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LRP1 85kDa subunit in C6, PC12, C2C12, SH-SY5Y cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Prolow-density lipoprotein receptor-related protein 1 (UniProt Q07954; also known as A2MR, Alpha-2-macroglobulin receptor, APER, Apolipoprotein E receptor, CD19. LRP-1) is encoded by the LRP1 (also known as A2MR, APR) gene (Gene ID: 4035) in human. LRP-1 is single pass type I membrane protein that is expressed in most tissues, but is abundant in liver, brain, and lung. It serves as a major VSMC receptor with a crucial role in maintaining vessel wall integrity and its expression in adult smooth muscle cells is shown to be essential for maintaining a contractile phenotype. LRP1 is synthesized with a signal peptide sequence (aa 1-19) and is processed in trans-Golgi network by Furin to generate a 515 kDa alpha subunit and an 85 kDa beta subunit that contains the 100-amino intracellular domain (LRPICD; aa 4445-4544). The alpha and beta subunits are non-covalently linked during LRP1 transport to the cell membrane. LRP1 recognizes and mediates the endocytosis of more than 40 different ligands, including apolipoprotein E (ApoE), APP and amyloid beta. It is the primary receptor mediating transport of amyloid beta peptides across the blood-brain barrier into circulation, thereby clearing them from the brain. LRP1 is required for early embryonic development and is involved in cellular lipid homeostasis and plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin). Excessive copper accumulation in the brain has been linked with reduced LRP1 mediated clearance of amyloid beta peptides across the blood brain barrier, which may contribute to complications of Alzheimer′s disease. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Au, DT et al. (2018). Arterioscler. Thromb. Vasc. Biol. 38(11); 2651- 2664).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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