W1878
Williams′ Medium E
With sodium bicarbonate, without ʟ-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
Pharma Manufacturing
Synonym(s):
Williams’ E medium
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
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Product Name
Williams′ Medium E, With sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: 0.025 g/L
phenol red: no
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L
L-glutamine: no
shipped in
ambient
storage temp.
2-8°C
General description
Williams′ Medium E was developed by Williams and Gunn as a modification to Williams′ Medium D. The medium is formulated to support long-term cell culture of adult liver epithelial cells. It is a modified Minimum Essential Medium (MEM) with altered glucose and amino acid content.
Application
Williams′ Medium E has been used in the culturing of human hepatocellular carcinoma (HCC) cells and hepatic cells.
Reconstitution
Supplement with 0.292 g/L L-glutamine.
Other Notes
Phenol red has been shown to interfere with the growth of some cells at low or cloning densities. Use this version of William's E when working with stem cells or cells at low densities.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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