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T3320

Sigma-Aldrich

Anti-γ-Tubulin (AK-15) antibody produced in rabbit

enhanced validation

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL number:
MFCD00677366
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 48 kDa

species reactivity

human, frog, rat

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunocytochemistry: 1:1,000 using methanol-acetone fixed, cultured rat, RAT-2 cells
microarray: suitable
western blot: 1:1,000 using whole cell extract of the human epitheloid carcinoma HeLa cell line.
western blot: 1:5,000 using Xenopus eggs extract

UniProt accession no.

P23258

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBG1(7283) , TUBG2(27175)
rat ... Tubg1(252921) , Tubg2(680991)

General description

γ-Tubulin (48 kDa) is a highly conserved protein. The microtubule cytoskeleton consists of a dynamic, highly polarized network of microtubules filaments, microtubule-associated proteins, microtubule motors, and microtubule-organizing proteins. γ-Tubulin is found as part of a large protein complex containing at least five other proteins and has a shape of a ring (γ-tubulin ring complex, γ-TuRC) that is roughly the same diameter as a microtubule.
γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ -Tubulin consists of two isoforms tubulinγ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ -tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

Application

Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in following studies:
  • Western blot.
  • Immunofluorescence.
Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in:
  • western blotting
  • immunocytochemistry (immunofluorescence staining
  • immunofluorescence

Biochem/physiol Actions

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression ofγ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The structure of the gamma-tubulin small complex: implications of its architecture and flexibility for microtubule nucleation
Kollman JM, et al.
Molecular Biology of the Cell, 19(1), 207-215 (2008)
L-Amino Acid Oxidase Isolated from B othrops pirajai Induces Apoptosis in BCR-ABL-Positive Cells and Potentiates Imatinib Mesylate Effect
Burin SM, et al.
Basic and Clinical Pharmacology and Toxicology, 113, 103-112 (2013)
l-Amino acid oxidase isolated from Calloselasma rhodostoma snake venom induces cytotoxicity and apoptosis in JAK2V617F-positive cell lines
Tavares C, et al.
Revista Brasileira de Hematologia e Hemoterapia, 38(2), 128-134 (2016)
Hakryul Jo et al.
PloS one, 5(4), e10318-e10318 (2010-04-30)
Microtubule drugs are effective anti-cancer agents, primarily due to their ability to induce mitotic arrest and subsequent cell death. However, some cancer cells are intrinsically resistant or acquire a resistance. Lack of apoptosis following mitotic arrest is thought to contribute
The gamma-tubulin gene family in humans.
Wise DO
Genomics, 67(2), 164-170 (2000)
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Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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