SRP6430

VEGF164 from mouse

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): VEGFA, VPF
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: mouse
• recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: calculated mol wt 19.3 kDa (no tag); observed mol wt 23-25 kDa (DTT-reduced. Protein migrates due to glycosylation. Ala 27 is the predicted N-terminal.)
• packaging: pkg of 10 μg
• UniProt accession no.: Q00731
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... VEGFA(22339)

Description

General description

Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable