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SAB5801061

Sigma-Aldrich

Rabbit Anti-Alpaca IgG2c Alpaca Heavy chain only Antibody

recombinant Alpaca(VHH) Nano antibody

Synonym(s):

Recombinant Alpaca single variable domain secondary Nanobody

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About This Item

UNSPSC Code:
12352203

biological source

alpaca

recombinant

expressed in HEK 293 cells (Human cell line)

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

recombinant monoclonal

form

lyophilized solid

mol wt

100 kDa

species reactivity

Alpaca, (alpaca)

technique(s)

ELISA: 1:4000-1:10000
western blot: 1:4000-1:10000

size

0.1 mL

color

colorless

isotype

VHH

shipped in

wet ice

storage temp.

2-8°C

General description

Rabbit Anti-Alpaca IgG2c Alpaca Heavy chain only Antibody is designed to specifically detect Alpaca IgG2c. It is a monovalent, recombinant single chain antibody fragment fused to rabbit IgG Fc. Based on immunoelectrophoresis and/or ELISA, Rabbit Anti-Alpaca IgG2c Alpaca Heavy chain only Antibody reacts with Alpaca IgG2c selectively, no cross-reactivity with Alpaca IgG1/2b.

The biological family Camelidae comprises camels (one-humped Camelus dromedarius and two-humped Camelus bactrianus), llama (Lama glama and Lama guanicoe), and vicugna (Vicugna vicugna and Vicugna pacos). Camelidae contain two kinds of IgG in serum: conventional antibodies (IgG1) containing two light chains and two heavy chains (composed of the VH, CH1, hinge, and CH2 and CH3 domains) and two types of homodimeric heavy-chain antibodies (HCAbs), IgG2 and IgG3, which comprise only H chains: each H chain contains a VHH, hinge, and CH2 and CH3 domains. The smallest intact functional antigen-binding fragment of HCAbs is the single-domain VHH, also known as a nanobody(Nb). Alpaca is also called Vicugna pacos. Alpaca IgG contains IgG1a, IgG1b, IgG2b, IgG2c and IgG3.

Specificity

Alpaca IgG2c
Isotype: Rabbit IgG

Immunogen

Recombinant Alpaca single variable domain secondary nano antibody

Packaging

100 μg in Glass bottle

Physical form

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Reconstitution

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Storage and Stability

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Other Notes

No cross-reactivity with Alpaca IgG1/2b

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317 - H412

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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