SAB4200594

Anti-APC (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Adenomatous polyposis coli protein, Anti-BTPS2, Anti-DP2, Anti-DP2.5, Anti-DP3, Anti-FAP, Anti-GS
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~300 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 3-6 μg/mL using lysates of HeLa or SW620 cells.; immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human colon or duodenum.; indirect immunofluorescence: 2-4 μg/mL using Caco-2 cells.
• UniProt accession no.: P25054
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... APC(324)

Description

General description

Adenomatous Polyposis Coli (APC) is a tumor suppressor gene. The APC gene encodes a large multifunctional protein that has critical functions in cell migration, adhesion and proliferation. It is a shuttling nucleo-cytoplasmic protein expressed in various tissues and its expression in the colorectal epithelium contributes to its normal growth and differentiation.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human APC, conjugated to KLH. The corresponding sequence is identical in human APC isoform b and highly conserved (single amino acid substitution) in rat and mouse APC.

Application

Anti-APC (C-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Biochem/physiol Actions

Adenomatous Polyposis Coli (APC) has a key role in the proteasome-mediated degradation of β-catenin. APC mutations cause accumulation of β-catenin in the nucleus leading to activation of LEF-1 and/or TCF, and the induction of target genes such as the oncogene c-myc. It is involved in regulation of mitotic chromosome separation and stability.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable