SAB4200534

Anti-Claudin-1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Claudin-1 (C-Terminal), antibody produced in rabbit, affinity isolated antibody
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~23 kDa
• species reactivity: human, dog
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: 20 μg/mL using formalin-fixed, paraffin-embedded human colon.; indirect immunofluorescence: 1.0-2.0 μg/mL using MDCK cells.; western blot: 0.5-1.0 μg/mL using extracts of HEK-293T cells overexpressing human claudin-1.
• UniProt accession no.: O95832
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CLDN1(9076)

Description

General description

Claudin-1, also known as claudin 1 (CLDN1), seminal metalloprotease-1 (Semp1) and ichthyosis, leukocyte vacuoles, alopecia, and sclerosing cholangitis (ILVASC), is expressed at high levels in kidney and liver and at lower levels in spleen, heart, brain, lung and testis. Claudins are characterized with four transmembrane domains and two extracellular loops, required to form tight junction strands.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminus of human claudin-1, conjugated to KLH. The corresponding sequence is highly conserved in mouse claudin-1 (single amino acid substitution) and in rat claudin-1 (90% identity).

Application

Anti-Claudin-1 (C-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Biochem/physiol Actions

Claudin-1 directly interacts with tight junction (TJ) -associated peripheral membrane proteins zonula occludens (ZO) proteins -1/2/3. Defects in the gene encoding claudin-1 are the cause of an autosomal recessive syndrome named ichthyosis-sclerosing cholangitis neonatal (NISCH). Claudins′ expression is altered in several human cancers. Claudin-1 is frequently up-regulated in colorectal carcinomas (CRCs), resulting in tumor differentiation and progression.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable