S8761

Sodium Bicarbonate

Solution (7.5%), sterile-filtered, suitable for cell culture

• Synonym(s): aqueous sodium bicarbonate
• CAS Number: 144-55-8
• MDL number: MFCD00003528
• UNSPSC Code: 12161702
• PubChem Substance ID: 24899815
• NACRES: NA.75

Properties

• product name: Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture
• sterility: sterile-filtered
• product line: BioReagent
• form: solution (7.5%)
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• pH: 7.8-8.2
• storage temp.: 2-8°C
• SMILES string: [Na+].OC([O-])=O
• InChI: 1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
• InChI key: UIIMBOGNXHQVGW-UHFFFAOYSA-M

Description

General description

Sodium bicarbonate (NaHCo₃) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process.

Application

Sodium bicarbonate solution has been used:

• as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice
• as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants
• as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma
• to prepare collagen mix droplets for 3D collagen sprouting assay
• to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture

Preparation Note

Prepared in cell culture grade water.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable