S5816

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse

SDF-1α, recombinant, expressed in E. coli, powder, suitable for cell culture

• Synonym(s): SDF-1α/PBSF
• MDL number: MFCD02100631
• UNSPSC Code: 12352202

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: >97% (SDS-PAGE)
• form: powder
• potency: 0.15-0.6 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: predicted mol wt ~8 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: <1 EU/μgtested (LAL test)
• UniProt accession no.: P40224
• storage temp.: −20°C
• Gene Information: mouse ... Cxcl12(20315)

Description

General description

Stromal derived factor-1 (SDF-1) is localized to adipocytes.

Application

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse has been used in invasion assay. It has also been used to study the SDF-1 release from chitosan-based nanoparticles.

Biochem/physiol Actions

SDF-1α/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. SDF-1α and SDF-1β (together also known as SDF-1α/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α..

Stromal derived factor-1 (SDF-1) is an insulin-desensitizing factor, upregulated during both starvation and obesity. Exogenic SDF-1 reduces insulin-mediated signaling and glucose uptake. SDF-1 mediates migration of cortical interneurons and endothelial progenitor cells of the bone marrow through chemotaxis.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

Preparation Note

Recombinant Mouse Stromal Cell-Derived Factor 1α (SDF-1α)/Pre-B Cell Growth Stimulating Factor (PBSF) is produced from a DNA sequence encoding the mature mouse SDF-1α protein sequence (amino acid residues 22-89) expressed in Escherichia coli. Recombinant mouse SDF-1α (68 amino acids) has a predicted molecular mass of approximately 8 kDa.

Analysis Note

The biological activity is measured by its ability to chemoattract cultured human lymphocytes and mouse BaF3 cells transfected with human CXCR4.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves