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Key Documents

P9614

Sigma-Aldrich

Lambda protein phosphatase

liquid, Bacteriophage Lambda, recombinant, expressed in E. coli

Synonym(s):

Lambda-PPase

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

form

liquid

mol wt

25 kDa

concentration

≥400000 units/mL

shipped in

dry ice

storage temp.

−70°C

General description

Lambda protein phosphatase is a recombinant protein expressed in Escherichia coli. It requires Mn2+ or Ni2+ as activators.

Biochem/physiol Actions

Lambda protein phosphatase has been used to dephosphorylate HeLa cell extracts.
Can be used to release phosphate groups from serine, threonine or tyrosine residues in proteins. Also active on phosphorylated histidine residues.

Unit Definition

One unit will hydrolyze 1 nanomole of p-nitrophenyl phosphate per min at pH 7.5 at 30 deg C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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