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P4399

Sigma-Aldrich

β-Lactamase from Enterobacter cloacae

Type III, lyophilized powder, 6-18 units/mg protein (using benzylpenicillin)

Synonym(s):

β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae

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About This Item

CAS Number:
9073-60-3
Enzyme Commission number:
3.5.2.6 (BRENDA, IUBMB)
MDL number:
MFCD00131819
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type III

form

lyophilized powder

specific activity

6-18 units/mg protein (using benzylpenicillin)

composition

Protein, ~10%

storage temp.

2-8°C

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Application

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4399 is produced from Enterobacter cloacae.

Biochem/physiol Actions

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.

Unit Definition

One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Physical form

Lyophilized powder containing sodium phosphate and sodium citrate buffer salts

Preparation Note

Chromatographically purified

Analysis Note

Protein determined by biuret

inhibitor

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sigma-Aldrich

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We present a new hypothesis for the selective pressures responsible for maintaining natural competence and transformation. Our hypothesis is based in part on the observation that in Bacillus subtilis, where transformation is widespread, competence is associated with periods of nongrowth
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Urinary tract infections (UTIs) are a common cause of bacteraemia in the elderly and are associated with a high probability of hospitalization. Despite the impact of UTIs on health status and quality of life, a limited number of studies have
A A Alsultan et al.
Journal of medical microbiology, 62(Pt 6), 885-888 (2013-03-23)
Carbapenem-resistant Acinetobacter baumannii is becoming increasingly prevalent in patients with diabetes mellitus in the Middle East. We examined the relationship of these bacteria and their resistance mechanisms to the diabetic disease status of patients in Saudi Arabia. Susceptibilities of 271
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