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P4039

Phospholipase C from Clostridium perfringens (C. welchii)

Name: Phospholipase C from <I>Clostridium perfringens (C. welchii)</I>
Brand: SIGMA

Type XIV, lyophilized powder, ≥150 units/mg protein

Synonym(s):

Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase

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About This Item

CAS Number:

9001-86-9

Enzyme Commission number:

3.1.4.3 (BRENDA, IUBMB)

EC Number:

232-638-2

MDL number:

MFCD00131895

UNSPSC Code:

41106300

NACRES:

NA.32

biological source

bacterial (Clostridium perfringens)

Quality Level

200

type

Type XIV

form

lyophilized powder

specific activity

≥150 units/mg protein

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative category

Enabling,

storage temp.

−20°C

Gene Information

Clostridium perfringens str. 13 ... plc(988262)

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.

Application

Phospholipase C from Clostridium perfringens has been used as a positive control in sandwich enzyme linked immunosorbent assay (ELISA) and ELISA.

Biochem/physiol Actions

Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.

Unit Definition

One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.

Physical form

Lyophilized powder in buffered salts

Preparation Note

Chromatographically purified

Pictograms

GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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In vitro inhibitory effect of hen egg white lysozyme on Clostridium perfringens type A associated with broiler necrotic enteritis and its alpha-toxin production

Zhang G, et al.

Letters in Applied Microbiology, 42(2), 138-143 (2006)

Sandwich ELISA detection of Clostridium perfringens cells and alpha-toxin from field cases of necrotic enteritis of poultry

McCourt MT, et al.

Veterinary Microbiology, 106(3-4), 259-264 (2005)

Development and evaluation of indirect enzyme-linked immunosorbent assays for the determination of immune response to multiple clostridial antigens in vaccinated captive bred southern white rhinoceros (Ceratotherium simum simum).

Angela Buys et al.

Acta veterinaria Scandinavica, 62(1), 57-57 (2020-10-09)

An overall increase in poaching of white rhinoceros results in captive breeding becoming a significant component of white rhinoceros conservation. However, this type of conservation comes with its own difficulties. When wildlife is captured, transported and/or confined to a boma

CD22 ligation inhibits downstream B cell receptor signaling and Ca(2+) flux upon activation.

N Sieger et al.

Arthritis and rheumatism, 65(3), 770-779 (2012-12-13)

CD22 is a surface molecule exclusively expressed on B cells that regulates adhesion and B cell receptor (BCR) signaling as an inhibitory coreceptor of the BCR. Central downstream signaling molecules that are activated upon BCR engagement include spleen tyrosine kinase

Sarcophine-diol inhibits expression of COX-2, inhibits activity of cPLA2, enhances degradation of PLA2 and PLC(γ)1 and inhibits cell membrane permeability in mouse melanoma B16F10 cells.

Pawel T Szymanski et al.

Marine drugs, 10(10), 2166-2180 (2012-11-22)

Sarcophine-diol (SD) is a semi-synthetic derivative of sarcophine with a significant chemopreventive effect against non-melanoma skin cancer both in vitro and in vivo. Recently, we have studied the effect of SD on melanoma development using the mouse melanoma B₁₆F₁₀ cell

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