P3375

Papain from papaya latex

crude powder, 1.5-10 units/mg solid

• Synonym(s): Papainase
• CAS Number: 9001-73-4
• Enzyme Commission number: 3.4.22.2 (BRENDA, IUBMB)
• EC Number: 232-627-2
• MDL number: MFCD00131791
• UNSPSC Code: 12352204
• eCl@ss: 42030311
• NACRES: NA.54

Properties

• form: crude powder
• specific activity: 1.5-10 units/mg solid
• mol wt: 23.4 kDa
• storage temp.: 2-8°C

Description

Application

Papain from papaya latex has been used:

• in the enzyme cocktail to evaluate the efficacy of B complex vitamers liberation from nutritional yeast powder
• in the enzyme mixture for the digestion of bovine milk samples for the analysis of three vitamin B₁ molecules
• as a biocatalyst for the hydrolysis of (R,S)-naproxen 2,2,2-trifluoroethyl ester in water-saturated solvents

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P3375, papain crude powder, has been used to digest fresh cartilage of dogsfish in order to isolate chondroitin sulfate.

Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Biochem/physiol Actions

Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Quality

Not standardized with lactose or other adulterants.

Unit Definition

One unit will hydrolyze one micromole of N-alpha-benzoyl-l-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.

Other Notes

View more information on papain at www.sigma-aldrich.com/enzymeexplorer.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves