M4159

Methylene Blue hydrate

suitable for nucleic acid staining, BioReagent

• Synonym(s): 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride xhydrate, Methylene Blue monohydrate, Methylene blue, Nucleic acid staining reagent
• Empirical Formula (Hill Notation): C₁₆H₁₈ClN₃S · xH₂O
• CAS Number: 122965-43-9
• Molecular Weight: 319.85 (anhydrous basis)
• Beilstein: 3599847
• EC Number: 200-515-2
• MDL number: MFCD00150006
• UNSPSC Code: 12171500
• PubChem Substance ID: 57654386
• NACRES: NA.52

Properties

• grade: for molecular biology
• product line: BioReagent
• form: powder
• technique(s): nucleic acid detection: suitable
• solubility: water: 1 mg/mL (dark blue)
• suitability: suitable for nucleic acid staining
• storage temp.: room temp
• SMILES string: O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
• InChI key: WQVSELLRAGBDLX-UHFFFAOYSA-M

Description

General description

Methylene blue is a heterocyclic aromatic chemical, appearing as a dark green solid powder, changing to blue in aqueous solution. It is used as a stain in many different histology procedures, as well as in molecular biology laboratories.

Application

Methylene Blue hydrate is suitable for use as a nucleic acid stain for agarose and polyacrylamide gel electrophoresis (PAGE). It was used to stain 4T1 tumor cells to study metastasis of tumor cells using clonogenic assay. Methylene blue hydrate crystals were encapsulated in apoferritin nanocages for photodynamic therapy against tumor cells.

Features and Benefits

• Less hazardous than staining with ethidium bromide
• Viewable in visible light
• Fast and easy to use

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves