M042030

Biotin Phosphoramidite

configured for ABI

• Synonym(s): Biotin amidite
• Empirical Formula (Hill Notation): C₄₆H₆₄N₅O₈PS
• CAS Number: 1217500-22-5
• Molecular Weight: 878.07
• MDL number: MFCD00676535
• UNSPSC Code: 41116105
• PubChem Substance ID: 329817780
• NACRES: NA.21

Properties

• product line: Proligo Reagents
• Assay: ≥96% (³¹P-NMR)
• suitability: conforms to structure for H-NMR
• compatibility: configured for ABI
• storage temp.: −20°C
• SMILES string: COc1ccc(cc1)C(N2C3CS[C@@H](CCCCC(=O)NCCOCCOCCOP(OCCC#N)N(C(C)C)C(C)C)C3NC2=O)(c4ccccc4)c5ccc(OC)cc5
• InChI: 1S/C46H64N5O8PS/c1-34(2)51(35(3)4)60(58-27-12-25-47)59-32-31-57-30-29-56-28-26-48-43(52)16-11-10-15-42-44-41(33-61-42)50(45(53)49-44)46(36-13-8-7-9-14-36,37-17-21-39(54-5)22-18-37)38-19-23-40(55-6)24-20-38/h7-9,13-14,17-24,34-35,41-42,44H,10-12,15-16,26-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,60?/m0/s1
• InChI key: WSDQYLGSIUJJRU-ZMKGMYJVSA-N

Description

General description

Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis. Biotin-labelled oligonucleotides have become extremely popular in DNAcapture and detection applications, due to their very high-sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotinbased detection methods and are widely used by researchers. Biotin labelling is compatible with PCR and most hybridization techniques.

Key Features of Biotin Phosphoramidite

• Behaves like any standard phosphoramidite on the DNA synthesizer
• Readily soluble in acetonitrile
• Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification
• A long, water-compatible linker arm ensures maximum sensitivity
• Provides a coupling efficiency of at least 95%The product is configured for ABI^™ Synthesizers

Legal Information

ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable