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L3768

Sigma-Aldrich

Lysing Enzymes from Aspergillus sp.

powder

Synonym(s):

Cellulase, Lysing Enzymes, Pectinase

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Aspergillus niger

form

powder

technique(s)

cell based assay: suitable

color

brown

suitability

suitable for cell lysis

application(s)

cell analysis

storage temp.

2-8°C

General description

Lysing enzymes are also known as lytic enzymes or cell wall lytic enzymes.

Application

Lysing Enzymes from Aspergillus sp. has been used to digest the cell walls to create pulse-field gel plugs.
Lysing Enzymes from Aspergillus sp. has been used:

  • to digest the cell wall of Schizosaccharomyces pombe
  • to lyse the mycelium cell wall of Trichoderma harzianum

Biochem/physiol Actions

Lysing enzymes are a group of enzymes that can break down the cell walls of microorganisms. These enzymes play a crucial role in various biological processes, including cell lysis, tissue dissociation, and protein extraction. Lysing enzymes are commonly used in research, biotechnology, and clinical applications. They are especially useful in DNA extraction, where they help to release genetic material from cells by breaking down the cell walls. This enables efficient isolation and purification of DNA for downstream analyses. Cellulases are a class of enzymes that degrade cellulose, a major component of plant cell walls, into simpler sugars. These sugars can be utilized as raw materials to produce biofuels, biobased chemicals, plastics, and various other materials. Pectinases are a group of enzymes that hydrolyze pectic substances found in microorganisms and higher plants. They can break down the pectin-containing compounds synthesized by plants and microbes.

Quality

Contains pectinase and cellulase activities.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tara L Mastro et al.
Journal of cell science, 133(10) (2020-04-23)
Translesion synthesis polymerases (TLSPs) are non-essential error-prone enzymes that ensure cell survival by facilitating DNA replication in the presence of DNA damage. In addition to their role in bypassing lesions, TLSPs have been implicated in meiotic double-strand break repair in
Julie M Bailis et al.
Molecular and cellular biology, 28(5), 1724-1738 (2008-01-09)
The minichromosome maintenance (MCM) complex plays essential, conserved roles throughout DNA synthesis: first, as a component of the prereplication complex at origins and, then, as a helicase associated with replication forks. Here we use fission yeast (Schizosaccharomyces pombe) as a

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