GE17-5478-51

HiTrap^® Protein L

Cytiva 17-5478-51, pack of 5 × 1 mL

• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: recombinant protein L (E. coli), mammalian free
• shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• packaging: pack of 5 × 1 mL
• manufacturer/tradename: Cytiva 17-5478-51
• storage condition: (20% Ehtanol)
• parameter: 5 bar
• column I.D.: 7 mm
• matrix: rigid, highly cross-linked agarose
• avg. part. size: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• working range: 2-10(pH interval where the medium can be operated without significant change in function.)
• suitability: suitable for bioprocess medium
• storage temp.: 2-8°C

Description

General description

HiTrap^® Protein L columns are packed with Capto^™ L affinity chromatography resin. It facilitates the purification of antibody fragments comprising the κ light chain. Capto^™ L resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which binds to the variable region of antibody κ light chains with strong affinity. This resin is an effective capture platform for antibody fragments having κ light chains due to its strong binding capacity and affinity to a wide spectrum of antibody fragments.

Application

HiTrap^® Protein L may be used to characterize human colorectal cancer MDR1/P-gp Fab and for the purification optimization of a single-chain variable fragment (scFv) against type 1 insulin-like growth factor receptor (IGF-1R). It may also be used for the purification of antigen-binding fragments (Fabs) and domain antibodies (dAbs).

Features and Benefits

• Captures a wide range of antibody fragments
• Allows a platform approach for the purification of antibody fragment
• Offers low ligand leakage, high binding capacity, and selectivity for a broad range of antibody fragments
• Reduces process time and amount of resin
• Suitable for process development and small-scale purification
• HiTrap^® format easily connects to a syringe, peristaltic pump, or chromatographic equipment, such as an ÄKTA^® system.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Capto is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids