GE17-5144-01

HiTrap^® Benzamidine Fast Flow

Cytiva 17-5144-01, pack of 1 × 5 mL

• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: p-aminobenzamidine
• packaging: pack of 1 × 5 mL
• manufacturer/tradename: Cytiva 17-5144-01
• storage condition: (0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)
• parameter: 42 psi
• bed size: 16 mm × 25 mm
• bed volume: 5 mL
• column I.D.: 16 mm
• matrix: highly cross-linked 4% agarose
• average diameter: 90 μm
• cleaning: 1-9
• working range: 2-8
• storage temp.: 2-8°C

Description

General description

HiTrap^® Benzamidine FF (high sub) are prepacked with Benzamidine Sepharose^™ 4 Fast FLow (high sub) and designed specifically for the removal and/or purification of serine proteases such as thrombin.

Application

HiTrap^® Benzamidine FF (high sub) are prepacked 1 mL and 5 mL columns for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.

The medium, Benzamidine Sepharose^™ 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC^™ System.

Features and Benefits

• Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.
• Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
• HiTrap^® Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
• High binding capacity.
• The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA^® design or FPLC^™ System.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

FPLC is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids