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GE17-1151-01

HiTrap® Sp High Performance

Cytiva 17-1151-01, pack of 5 × 1 mL

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

sulphopropyl

description

Ion Exchanger Type (value)

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-1151-01

parameter

flow rate
42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

6% cross-linked agarose

particle size

24-44 μm

avg. part. size

34 μm

cleaning

3-14

working range

4-13

suitability

suitable for bioprocess medium

General description

HiTrap® SP HP are prepacked, ready-to-use SP Sepharose High Performance strong cation exchange columns for high-resolution, small-scale protein purification.

The small particle size (34μm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose High Performance is a strong cation exchange medium and Q Sepharose High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.

Features and Benefits

  • 34 μm bead size for high-performance, high-resolution purifications.
  • Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
  • Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
  • Strong sulfopropyl (SP) cation exchanger.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows how to perform a purification of His-tagged membrane proteins with products from GE Healthcare.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

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Protocols

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service