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Key Documents

MOP-8

92111707, mouse embryo, Fibroblast

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About This Item

UNSPSC Code:
41106514

product name

MOP-8, 92111707

biological source

mouse embryo

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse, SV40 Polyoma transformed NIH/3T3 cells

Cell Line Description

MOP-8 was developed by transforming NIH/3T3 cells with a hybrid transcription unit composed of the SV40 early promoter fused to the early region of polyomavirus. The cells should be handled under laboratory containment level 2.

Application

Suitable host for transfection studies, polyoma virus replication studies

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA, 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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