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product name
LL47 (MaDo), 90102538
biological source
human lung
growth mode
Adherent
karyotype
2n = 46; diploid
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human diploid lung
Cell Line Description
Derived from normal lung tissue obtained from a 16 year old male with osteogenic sarcoma. Ethnicity: Black.
Application
Tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,12
D13S317: 9,11
D16S539: 11
D5S818: 12,13
D7S820: 8,10
THO1: 7,9.3
TPOX: 9,10
vWA: 14,16
CSF1PO: 7,12
D13S317: 9,11
D16S539: 11
D5S818: 12,13
D7S820: 8,10
THO1: 7,9.3
TPOX: 9,10
vWA: 14,16
Culture Medium
Coon′s modified Ham′s F12 + Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. A further 15 population doublings expected.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
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