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product name
SW 1116, 87071006
biological source
human colon
growth mode
Adherent
karyotype
2n = 46, hypotriploid
morphology
Epithelial
products
Carcinoembryonic antigen (CEA)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian colon adenocarcinoma
Cell Line Description
Derived from a grade II adenocarcinoma of the colon extending into the muscularis from a 73 year old Caucasian male. The cells exhibit brush borders and produce high levels of CEA.
Application
Tumourigenicity studies
Culture Medium
L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; CO2 is not needed. Medium change after 4-5 days. 37°C.
Other Notes
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Certificates of Analysis (COA)
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