MIA-Pa-Ca-2

85062806

• Synonym(s): MIA PaCa-2 Cells, MIA-PaCa2 Cells, MIAPACA-2 Cells, PaCa2 Cells
• UNSPSC Code: 41106514

Properties

• biological source: human pancreas
• growth mode: Adherent
• karyotype: Modal no. 61; hypotriploid
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian pancreatic carcinoma

Cell Line Description

An established cell line from an undifferentiated human pancreatic carcinoma. The tumour was taken from a 65 year old Caucasian male. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy, have a tendency to grow on top of other cells, eventually growing free in suspension. Sensitive to L-Asparaginase.

Application

MIA-Pa-Ca-2 has been used for the assessment of thyroid receptor transcriptional activity and for α-enolase immunostaining.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 10,13
D5S818: 12,13
D7S820: 12,13
THO1: 9,10
TPOX: 9
vWA: 15

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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