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biological source
Mouse x Mouse Hybridoma
ImmunoDon
Balb/c mouse spleen
Myeloma
NS0 mouse myeloma
Reactivity
Rat IgG2b heavy chain
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
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Cell Line Origin
Monoclonal antibody rat IgG2b
Cell Line Description
Mouse hybridoma against rat IgG2b heavy chain. Cambridge Enterprise Reference number AB71:www.enterprise.cam.ac.uk.
Immunogen
Rat IgG
Culture Medium
IDMEM (I3390) + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442). Where the cells are grown under static conditions, 0.02% EDTA, Trypsin (T4424) or mechanical agitation maybe required to dislodge them from the plastic.
Subculture Routine
Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 Ā°C. Saturation density 9 x 105/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ā²Cell Line Release Authorisation for Research Use in Commercial Organisationsā² release conditions form.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a productās label following the words āLotā or āBatchā.
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