KARPAS 422
06101702, human lymph (lymphoma). Round polygonal singlets or small clusters in suspension
Synonym(s):
KARPAS, 422
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About This Item
UNSPSC Code:
41106514
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product name
KARPAS 422, from human lymph(lymphoma), 06101702
biological source
human lymph (lymphoma)
growth mode
Suspension
karyotype
Hyperdiploid with 10% polyploidy
morphology
Round polygonal cells growing singly or in small clusters in suspension
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Cell Line Origin
Human B cell non-Hodgkin lymphoma
Cell Line Description
Established from a pleural effusion of 73 year-old woman diagnosed with B-cell non-Hodgkin lymphoma (intra-abdominal, diffuse large cell lymphoma, refractory, terminal). Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).
DNA Profile
Not specified
Culture Medium
RPMI 1640 + 2 mM Glutamine + 20% Fetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 0.5 - 2 x 1000,000 cells/ml; 5% CO2; 37 °C. Doubling time approximately 60-90 hours. Split saturated culture 1:2 every 2-4 days. Round polygonal cells growing singly or in small clusters in suspension.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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