MDCK-SIAT1 Cell Line canine

5071502, canine kidney, Epithelial

• Synonym(s): MDCK-SIAT1 Cells
• UNSPSC Code: 41106514

Properties

• Product Name: MDCK-SIAT1 Cell Line canine, 05071502
• biological source: canine kidney
• description: Canine Cocker Spaniel Kidney Sialic Acid Over Expression
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Enhanced expression of 6-linked sialic acids
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Canine Cocker Spaniel Kidney Sialic Acid Over Expression

Cell Line Description

This cell line has been derived by the stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). The cells express two fold?higher amounts of 6-linked sialic acids and two fold-lower amounts of 3-linked sialic acids than parent MDCK cells. MDCK-SIAT1 cells are presented as a suitable system for testing the sensitivity of human influenza virus to neuraminidase inhibitors (NAI) due to their over expression of sialyl-α2,6-galactose moieties.

Culture Medium

DMEM + 2mM Glutamine + 1mg/ml G418 + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (85-95 %) 1:10 to 1:20 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO₂.

Other Notes

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