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Key Documents

C4585

Sigma-Aldrich

Anti-β-Tubulin−Cy3 antibody, Mouse monoclonal

clone TUB 2.1, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-β-Tubulin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

CY3 conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

TUB 2.1, monoclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:100 using cultured chicken fibroblasts or BHK cells

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... TUBB(203068) , TUBB1(81027) , TUBB1(81027) , TUBB1(81027) , TUBB1(81027) , TUBB2A(7280) , TUBB2A(7280) , TUBB2A(7280) , TUBB2A(7280) , TUBB2C(10383) , TUBB2C(10383) , TUBB2C(10383) , TUBB2C(10383)
mouse ... Tubb1(104068) , Tubb1(104068) , Tubb1(104068) , Tubb1(104068) , Tubb2a(22151) , Tubb2a(22151) , Tubb2a(22151) , Tubb2a(22151) , Tubb2c(227613) , Tubb2c(227613) , Tubb2c(227613) , Tubb2c(227613) , Tubb3(22152)
rat ... Tubb2(29212) , Tubb2(29212) , Tubb2(29212) , Tubb2(29212) , Tubb2c(296554) , Tubb2c(296554) , Tubb2c(296554) , Tubb2c(296554)

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General description

Monoclonal Anti-b-Tubulin (mouse IgG1 isotype) is derived from the TUB2.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified rat brain tubulin. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. β-tubulin structure is characterized with the core of two β-sheets enclosed by α-helices.

Specificity

The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Immunogen

tubulin from rat brain.

Application

Monoclonal Anti-β-Tubulin-Cy3 antibody produced in mouse has been used in:
  • immunohistochemical assays
  • immunofluorescence
  • western blotting
  • immunocytochemistry
  • immunoblotting
  • immunostaining

Biochem/physiol Actions

Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Mutation in the β-tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Liang Qiang et al.
Molecular biology of the cell, 21(2), 334-344 (2009-11-27)
The formation of interstitial axonal branches involves the severing of microtubules at sites where new branches form. Here we wished to ascertain whether basic fibroblast growth factor (bFGF) enhances axonal branching through alterations in proteins involved in the severing of
The microtubule-severing proteins spastin and katanin participate differently in the formation of axonal branches
Yu W, et al.
Molecular Biology of the Cell, 19(4), 1485-1498 (2008)
TAPping into the treasures of tubulin using novel protein production methods
Yu N and Galjart N
Essays in Biochemistry, 62(6), 781-792 (2018)
M Janmaleki et al.
Scientific reports, 6, 32418-32418 (2016-09-02)
This study focused on the effects of simulated microgravity (s-μg) on mechanical properties, major cytoskeleton biopolymers, and morphology of endothelial cells (ECs). The structural and functional integrity of ECs are vital to regulate vascular homeostasis and prevent atherosclerosis. Furthermore, these
Comprehensive genotype-phenotype correlation in lissencephaly
Tan AP, et al.
Quantitative imaging in medicine and surgery, 8(7), 673-673 (2018)
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Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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