B1434

Bone Morphogenetic Protein 7 human

>95% (SDS-PAGE), recombinant, expressed in CHO cells, lyophilized powder, suitable for cell culture

• Synonym(s): BMP-7, OP-1, Osteogenic protein 1
• MDL number: MFCD03702812
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in CHO cells
• Assay: >95% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.2-0.6 μg/mL ED₅₀
• mol wt: monomer calculated mol wt 15.7 kDa; 18-20 kDa by SDS-PAGE (reducing); 30-40 kDa by SDS-PAGE (non-reducing)
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P18075
• storage temp.: −20°C
• Gene Information: human ... BMP7(655)

Description

Biochem/physiol Actions

Cellular responses to BMP-7 are mediated by the formation of hetero-oligomeric complexes of type I and II serine/threonine kinase receptors. Human and mouse BMP-7 share 98% amino acid sequence identity.

Physical form

Lyophilized from 50.0 μl of a 0.2 μm filtered solution in 35% acetonitrile, 0.1% TFA containing 50 μg bovine serum albumin per 1μg of cytokine.

Analysis Note

The biological activity is measured by its ability to induce alkaline phosphatase production using mouse ATDC-5 chondrogenic cells.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319
• Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves