A2765
Azocasein
protease substrate
Synonym(s):
Sulfanilamide-azocasein
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About This Item
Recommended Products
biological source
bovine
Quality Level
form
powder
technique(s)
ligand binding assay: suitable
solubility
water: 5 mg/mL, clear, orange to very deep orange
ε (extinction coefficient)
≥25 at 440 nm in 0.1 M NaOH at 1%
storage temp.
2-8°C
General description
Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.
Application
Azocasein has been used as a substrate for determination of protease activity.
Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.
Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.
Linkage
View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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