646563
DL-Dithiothreitol solution
1 M in H2O
Synonym(s):
(±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT
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About This Item
Empirical Formula (Hill Notation):
C4H10O2S2
CAS Number:
Molecular Weight:
154.25
Beilstein:
1719757
MDL number:
UNSPSC Code:
12161504
PubChem Substance ID:
NACRES:
NA.25
Recommended Products
form
liquid
Quality Level
reaction suitability
reagent type: reductant
concentration
1 M in H2O
density
1.04 g/mL at 20 °C
1.035 g/mL at 25 °C
SMILES string
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key
VHJLVAABSRFDPM-QWWZWVQMSA-N
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General description
Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.
Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.
Application
DL-Dithiothreitol solution has been used:
- in western blot analysis
- as a supplement in lysis buffer to study its effects on sperm lysis
- as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues
- in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds
Features and Benefits
Low toxicity and reduced odor compared to other thiol compounds.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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