11089161001

Streptavidin-AP conjugate

solution, pkg of 1 mL (1,000U)

• Synonym(s): streptavidin
• UNSPSC Code: 12352200

Properties

• form: solution
• Quality Level: 100
• packaging: pkg of 1 mL (1,000U)
• manufacturer/tradename: Roche
• storage temp.: 2-8°C

Description

General description

Streptavidin is covalently coupled to alkaline phosphatase (AP) for the preparation of streptavidin-AP-conjugate.

Alkaline Phosphatase
Alkaline Phosphatase is often used as a label in enzyme immunoassays. AP converts the sensitive substrate X-phosphate/NBT into a stable, water soluble product.

Application

Streptavidin-AP has been used to detect biotinylated compounds using:

• ELISA
• Immunohistocytochemistry
• Western blot

Physical form

Solution, stablilized in 50 mM Triethanolamine buffer, 3 M NaCl, 1 mM MgCl₂, 0.1 mM ZnCl₂, 10 mg/ml bovine serum albumin, pH 7.6.

Preparation Note

Working solution: 50 - 1,000 mU/ml (i.e., stock solution can be diluted). Dilute the conjugate stock solution in 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.

Working concentration: Working concentration of conjugate will depend on the application and substrate. Indicated concentrations should be taken as a guideline.

ELISA: 50 - 200 mU/ml (1:5000 - 1:20,000 dilution)
Immunohisto/cytochemistry: 250 - 1,000 mU/ml (1:1000 - 1:4000 dilution)
Western blot: 200 - 500 mU/ml (1:2000 - 1:5000 dilution)

Stability: The concentrated stock solution is stable when stored at 2 to 8°C until the expiration date printed on the label. Do not freeze!

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): No data available
• Flash Point(C): No data available