MABT820

Anti-Integrin alpha 5 (CD49e) Antibody, clone mAb16

clone mAb16, from rat

• Synonym(s): CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: mAb16, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_002196
• UniProt accession no.: P08648
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... ITGA5(3678)

Description

General description

Integrin alpha-5 (UniProt: P08648; also known as CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e) is encoded by the ITGA5 (also known as FNRA) gene (Gene ID: 3678) in human. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. Integrin alpha-5 is a single-pass type I membrane protein is expressed on multiple cell types. It contains four calcium binding sites (aa 480-488; 334-342; 401-409; and 465-473). Structurally, it consists of an extracellular domain (aa 4-995), a transmembrane domain (aa 996-1021), and a cytoplasmic domain (aa 1022-1049). When associated with beta 1 subunit it can serve as a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. Integrin alpha-5 is also expressed in articular cartilage and plays a role in synovial joint development and in the regulation of chondrocyte survival and matrix degradation in articular cartilage. Membrane fractions obtained from fibroblasts of diabetic animals display significant reduction in integrin alpha-5 levels. (Ref.: Almeida, ME et al., (2016). Mol. Cell. Biochem. 421(1-2); 19-28).

Specificity

Clone mAb16 is a rat monoclonal antibody that specifically detects Integrin alpha5 in human cells.

Immunogen

Fibronectin receptor purified from human placenta.

Application

Anti-Integrin alpha5 (CD49e), clone mAb16, Cat. No. MABT820, is a rat monoclonal antibody that detects Integrin alpha5 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Inhibition assays.

Inhibition Analysis: 10 ug/mL from a representative lot prevented HT-1080 cells from adhering to fibronectin coated plates (Courtesy of Dr. Kenneth M. Yamada, M.D., Ph.D., NIDCR, National Institutes of Health, Bethesda, MD USA).

Inhibition Analysis: A representative lot inhibited WI38 fibroblast attachment to fibronectin (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).

Inhibition Analysis: A representative lot inhibited A375-SM cell spreading. (Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43).

Immunocytochemistry Analysis: A representative lot detected Integrin alpha5 (CD49e) in Immunocytochemistry applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).


Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin alpha5 (CD49e) in Immunoprecipitation applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).

Research Category
Cell Structure

Quality

Evaluated by Immunocytochemistry in HFF cells.

Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected Integrin alpha5 (CD49e) in human Foreskin Fibroblast (HFF) cells.

Target description

114.54 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2a in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2