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MABS487

Sigma-Aldrich

Anti-Modified Citrulline Antibody, clone C4

clone C4, from human(Recombinant)

Synonym(s):

citrulline, modified citrulline

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

human (Recombinant)

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

C4, monoclonal

species reactivity (predicted by homology)

all

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG1λ

shipped in

wet ice

target post-translational modification

unmodified

General description

Citrulline is thought to be an unincorporated amino acid that plays a part in intermediary metabolism. It is produced in the small intestine by enterocytes. Citrulline concentration in plasma and serum is thought to be a reliable and noninvasive biomarker of small intestine function and mass during disease. Citrulline has also been shown to play an important role in protein homeostasis. Modified citrulline is a chemically modified form of citrulline that is more immunogenic.

Immunogen

Citrulline-containing peptide modified with 2,3-butanedione monoxime and antipyrine.

Application

Research Category
Signaling
Research Sub Category
Signaling Neuroscience
This Anti-Modified Citrulline Antibody is validated for use in Western blotting and ELISA for the detection of Modified Citrulline.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Modified Citrulline in 10 µg of treated HL-60 cell lysate, using Anti-Human secondary antibody.
ELISA Analysis: A representative lot detected Modified Citrulline in ELISA.

Quality

Evaluated by Western Blotting in treated cornified human cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Modified Citrulline in 10 µg of treated cornified human cell lysate, using Anti-Human secondary antibody.

Physical form

Format: Purified
Human recombinant monoclonal IgG1λ in buffer containing PBS with 0.05% sodium azide.
Protein A purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Seema Qamar et al.
Cell, 173(3), 720-734 (2018-04-21)
Reversible phase separation underpins the role of FUS in ribonucleoprotein granules and other membrane-free organelles and is, in part, driven by the intrinsically disordered low-complexity (LC) domain of FUS. Here, we report that cooperative cation-π interactions between tyrosines in the
Charlotte Thålin et al.
Journal of thrombosis and haemostasis : JTH, 18(10), 2732-2743 (2020-07-13)
Recent data propose a diagnostic and prognostic capacity for citrullinated histone H3 (H3Cit), a marker of neutrophil extracellular traps (NETs), in pathologic conditions such as cancer and thrombosis. However, current research is hampered by lack of standardized assays. We aimed

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