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Key Documents

MABF160A

Sigma-Aldrich

Anti-CD45 (human), PerCP-Cy5.5, clone HI30 Antibody

clone HI30, from mouse, PerCP-Cy5.5

Synonym(s):

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), Lymphocyte antigen 5 (Ly-5), T200, CD45, CD45

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44

biological source

mouse

Quality Level

100

conjugate

PerCP-Cy5.5

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

HI30, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable

UniProt accession no.

P06800

target post-translational modification

unmodified

Gene Information

human ... PTPRC(5788)

General description

The HI30 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.The HI30 antibody is widely used as a marker for human CD45 expression on T cells, B cells, monocytes, macrophages, and NK cells.

Application

Anti-CD45 (human), PerCP-Cy5.5, clone HI30 Antibody is an antibody against CD45 for use in Flow Cytometry.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected CD45 in one million human peripheral blood lymphocytes.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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