MAB353B

Anti-Nestin Antibody, clone rat-401, Biotin Conjugate

clone rat-401, from mouse, biotin conjugate

• Synonym(s): rat401, NES, Nbla00170, FLJ21841
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: biotin conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: rat-401, monoclonal
• species reactivity: mouse, rat
• should not react with: human
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable
• isotype: IgG1
• NCBI accession no.: NP_037119
• UniProt accession no.: P21263
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NES(10763) ; mouse ... Nes(18008) ; rat ... Nes(25491)

Description

General description

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

Specificity

This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.

Immunogen

Embryonic rat spinal cord

Application

Detect Nestin using this mouse monoclonal antibody, Anti-Nestin Antibody, clone rat-401, Biotin Conjugate validated for use in ICC & IHC.

Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022).
Immunocytochemsitry Analysis: A 1:200 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).
Evaluated by Immunocytochemistry in ReNcell VM human neural progenitor cells (SCC008).
Immunocytochemsitry Analysis: A 1:200 dilution of this antibody did not detect Nestin in ReNcell VM human neural progenitor cells (SCC008). Evaluated by Immunohistochemistry in rat hindbrain brain tissue.
Immunohistochemsitry Analysis: A 1:50 dilution of this antibody detected Nestin in rat hindbrain brain tissue.

Research Category
Stem Cell Research

Neuroscience

Research Sub Category
Developmental Neuroscience

Neural Stem Cells

Quality

Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:200 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).

Target description

200-220 kDa calculated

Physical form

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA.

Storage and Stability

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Mouse cortical neural stem cells (SCR029)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable